iGEM

iGEM
To verify the construction of xynA-xynD, the digestion and agarose gel electrophoresis were performed by a standard protocol.
Moreover, Acidic Xylanase Assay (DNS demonstration method) was used to determine the enzyme activity of xylanase.
As shown in Fig. 2B, the xylanase was produced as expected, and the enzyme activity is 0.105 U/mL (Definition of enzyme activity)
iGEM
1 unit, the amount of enzyme required to hydrolysis
xylan to produce 1 μmol of reducing sugar per
milliliter of fermentation broth, under the condition of 50℃ and pH 4.8
To verify the construction of xynA-xynD, the digestion and agarose gel electrophoresis were performed by a standard protocol.
Moreover, Acidic Xylanase Assay (DNS demonstration method) was used to determine the enzyme activity of xylanase.
As shown in Fig. 2B, the xylanase was produced as expected, and the enzyme activity is 0.105 U/mL (Definition of enzyme activity)
iGEM
1 unit, the amount of enzyme required to hydrolysis
xylan to produce 1 μmol of reducing sugar per
milliliter of fermentation broth, under the condition of 50℃ and pH 4.8
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